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Stephen Johnston

Stephen.Johnston@asu.edu

Professor
Biodesign Cim
Faculty
Mail Code: 5901

BDB 230A (Map)
(480)727-0792

Bio

Director, Center for Innovations in Medicine, Biodesign Institute

Director, Biological Design Graduate Program

Professor
School of Life Sciences
 

Stephen Johnston is currently the Director for the Center for Innovations in Medicine (CIM), a Professor in the School of Life Sciences, and Director of the Biological Design Graduate Program at The Biodesign Institute at Arizona State University.  The CIM's and Dr. Johnston's current work focuses on innovative solutions to fundamental problems in biomedicine.  The CIM is unique in that it has brought together a group of interdisciplinary scientists who first identify a problem, analyze the physical economy basis of the related issues and then come up with an inventive solution.  Current major projects are 1) Cancer Eradication: The focus is on developing a universal, preventative cancer vaccine, 2) Health Futures: The aim is to produce a diagnostic system that allows  continuous monitoring of the health status of healthy people - helping in the revolution to pre-symptomatic medicine.   Dr. Johnston has experience in basic science, notably first cloning the Gal4 gene, showing that proteins have separable functional domains, and discovering the AAA proteins and their role in transcription.  His focus now is in translational sciences and technology development.  He was co-inventor/innovator of pathogen derived resistance, organelle transformation, the gene gun, genetic immunization, TEV protease system, expression library immunization, linear expression elements, synbodies and immunosignaturing.  He is author of over 150 journal articles, has over 20 patents, and has garnered approximately $85M in grant support including large programs from DARPA, NIAID and NHLBI.  

Expertise

Drug targeting - vaccine technology - cancer treatment - presymptomatic diagnosis of cancers through identifying the biosignatures of disease

Education

1981, Ph.D. in Genetics and Plant Genetics/Plant Breeding, University of Wisconsin/Madison

 Recent Publications

 

Berea A. R. Williams, Chris W. Diehnelt, Paul Belcher, Matthew Greving, Neal W. Woodbury, Stephen A. Johnston, and John C. Chaput. Creating Protein Affinity Reagents by Combining Peptide Ligands on Synthetic DNA Scaffolds. Journal of the American Chemical Society. 10.1021/ja9051735. 6 November 2009.

Boltz KW, Gonzalez-Moa MJ, Stafford P, Johnston SA, Svarovsky SA. 2009. Peptide microarrays for carbohydrate recognition. Analyst. Apr;134(4):650-2.

Morales Betanzos C, Gonzalez-Moa MJ, Boltz KW, Vander Werf BD, Johnston SA, Svarovsky SA. 2009. Bacterial glycoprofiling by using random sequence peptide microarrays. Chembiochem. Mar 23;10(5):877-88.

Betanzos CM, Gonzalez-Moa M, Johnston SA, Svarovsky SA. 2009. Facile labeling of lipoglycans with quantum dots. Biochem Biophys Res Commun. Feb 27;380(1):1-4.

Ferdous A, O'Neal M, Nalley K, Sikder D, Kodadek T, Johnston SA. 2008. Phosphorylation of the Gal4 DNA-binding domain is essential for activator mono-ubiquitylation and efficient promoter occupancy. Mol Biosyst. Nov;4(11):1116-25.

Archer, Chase T ; Delahodde, Agnes ; Gonzalez, Fernando ; Johnston, Stephen Albert ; Kodadek, Thomas. 2008. Activation domain-dependent monoubiquitylation of Gal4 protein is essential for promoter binding in vivo The Journal of biological chemistry. Vol 283. No 18. 12614-23

Kumar, P.K., Y. Yu, R. Sternglanz, S. A. Johnstonand L. Joshua-Tor. 2008. NADP regulates the yeast GAL induction system. Science. 319: 1090-1092

Qu, B., Q. Xiang, L. Li, S.A.Johnston, L.S. Hynan and R. N. Rosenberg. 2007. AB42 Gene vaccine prevents AB42 deposition in brain of double transgenic mice. J. Neurological Sci. 260: 204-213.

Johnston, S.A. 2007. The Potential Importance of Presymptomatic, Host-Based Diagnosis in Biodefence and Standard Health Care. In: Global Infectious Disease Surveillance and Detection. National Academy Press. p193-212.

Anwarul Ferdous, Devanjan Sikder, Thomas Gillette, Kip Nalley, Thomas Kodadek, and Stephen Albert Johnston. 2007. The role of the proteasomal ATPases and activator monoubiquitylation in regulating Gal4 binding to promoters. Genes & Dev. 21: 112-123. (Published in Advance December 13, 2006, 10.1101/gad.1493207)

Courses

Spring 2014

Courses Taught This Semester
Course Number Course Title
BDE 792 Research
BDE 799 Dissertation
MIC 493 Honors Thesis

Fall 2013

Courses Taught This Semester
Course Number Course Title
BDE 792 Research
BDE 799 Dissertation

Spring 2013

Courses Taught This Semester
Course Number Course Title
BIO 495 Undergraduate Research

Spring 2012

Courses Taught This Semester
Course Number Course Title
BIO 499 Individualized Instruction
MIC 495 Undergraduate Research

Fall 2011

Courses Taught This Semester
Course Number Course Title
BIO 495 Undergraduate Research

Summer 2011

Courses Taught This Semester
Course Number Course Title
BIO 499 Individualized Instruction

Spring 2011

Courses Taught This Semester
Course Number Course Title
MIC 499 Individualized Instruction

Fall 2010

Courses Taught This Semester
Course Number Course Title
BIO 499 Individualized Instruction

Publications and Other Intellectual ContributionsGoogle Scholar

  1. Domenyuk V, Loskutov A, Johnston SA, Diehnelt CW. A Technology for Developing Synbodies with Antibacterial Activity. PLoS ONE (2013).

  2. Legutki JB, Johnston SA. Immunosignatures can predict vaccine efficacy. PNAS (2013).

  3. Johnston, s.a.. Cancer goal: vaccine is cause for optimism. Nature (2013).

  4. Halperin RF, Stafford P, Emery JS, Navalkar KA, Johnston SA. GuiTope: An Application for Mapping Random-Sequence Peptides to Protein Sequences. BMC Bioinformatics (2012).

  5. Hughes AK, Cichacz Z, Scheck A, Coons SW, Johnston SA, Stafford P. Immunosignaturing can detect products from molecular markers in brain cancer. PLoS One (2012).

  6. Kroening K, Johnston SA, Legutki JB. Autoreactive antibodies raised by self derived de novo peptides can identify unrelated antigens on protein microarrys.. Experimental and Molecular Pathology (2012).

  7. Kukreja M, Johnston SA, Stafford P. Comparative study of classification algorithms for immunosignaturing data. BMC Bioinformatics (2012).

  8. Kukreja m, Johnston SA, Stafford P. Immunosignaturing Microarrays Distinguish Antibody Profiles of Related Pancreatic Diseases. Proteomics & Bioinformatics (2012).

  9. McGuire, MN., Johnston, SA., Sykes, KE. Novel immune-modulator identified by a rapid, functional screen of the Parapoxvirus ovis (Orf virus) genome.. Proteome Science (2012).

  10. Restrepo L, Stafford P, Johnston SA. Feasibility of an Early Alzheimer's Disease Immunosignature Diagnostic Test. Journal of Neuroimmunology (2012).

  11. Stafford, P., Halperin, R., Legutki, JB., Magee, DM., Galgiani, J., Johnston, SA. Physical Characterization of the "Immunosignaturing Effect". Molecular Cell Proteomics (2012).

  12. Brown, J., Stafford, P., Johnston, SA, Dinu, V.. Statistical Methods for Analyzing Immunosignatures. BMC Bioinformatics (2011).

  13. Gupta N., Belcher P., Johnston SA., Diehnelt C. Engineering a Synthetic Ligand for Tumor Necrosis Factor-Alpha. Bioconjugate Chemistry (2011).

  14. Restrepo L., Stafford P., Magee DM., Johnston SA. Application of immunosignatures to the Assessment of Alzheimer's disease. Annals of Neurology (2011).

  15. Stafford P, Johnston SA. Microarray technology displays the complexities of the humoral immune response. Expert Reviews in Molecular Diagnostics (2011).

  16. Diehnelt C, Shah, M, Gupta, N, Belcher, PE, Greving, MP, Stafford, P, Johnston, SA. Discovery of High-Affinity Protein Binding Ligands - Backwards. PLoS One (2010).

  17. Fu J, Cai K, Johnston SA, Woodbury NW. Exploring peptide space for enzyme modulators. J Am Chem Soc (2010).

  18. Greving MP, Belcher PE, Diehnelt CW, Gonzalez-Moa MJ, Emery J, et al.. Thermodynamic Additivity of Sequence Variations: An Algorithm for Creating High Affinity Peptides Without Large Libraries or Structural Information. . PLoS ONE (2010).

  19. Legutki JB, Magee DM, Stafford P, Johnston SA. A general method for characterization of humoral immunity induced by a vaccine or infection. Vaccine (2010).

  20. Berea A. R. Williams, Chris W. Diehnelt, Paul Belcher, Matthew Greving, Neal W. Woodbury, Stephen A. Johnston, and John C. Chaput. Creating Protein Affinity Reagents by Combining Peptide Ligands on Synthetic DNA Scaffolds. Journal of the American Chemical Society (2009).

  21. Betanzos CM, Gonzalez-Moa M, Johnston SA, Svarovsky SA. Facile labeling of lipoglycans with quantum dots. Biochem Biophys Res Commun (2009).

  22. Boltz KW, Gonzalez-Moa MJ, Stafford P, Johnston SA, Svarovsky SA. Peptide microarrays for carbohydrate recognition.. Analyst. (2009).

  23. Morales Betanzos C, Gonzalez-Moa MJ, Boltz KW, Vander Werf BD, Johnston SA, Svarovsky SA. Bacterial glycoprofiling by using random sequence peptide microarrays. Chembiochem (2009).

  24. Archer, Chase T ; Delahodde, Agnes ; Gonzalez, Fernando ; Johnston, Stephen Albert ; Kodadek, Thomas.. Activation domain-dependent monoubiquitylation of Gal4 protein is essential for promoter binding in vivo. The Journal of biological chemistry (2008).

  25. Kumar, P.K., Y. Yu, R. Sternglanz, S. A. Johnston and L. Joshua-Tor. NADP regulates the yeast GAL induction system.. Science. (2008).

  26. Talaat AM, Ward SK, Wu CW, Rondon E, Tavano C et al.. Mycobacterial bacilli are metabolically active during chronic tuberculosis in murine lungs: Insights from genome-wide transcriptional profiling. Journal of Bacteriology (2007).

  27. B Qu, P Boyer, Stephen Johnston, L Hynan, R Rosenberg. Abeta-42 gene vaccination reduces brain amyloid plaque burden in transgenic mice. J. Neurol. Sci (2006).

  28. J Lawson, R Lyons, Stephen Johnston. Expression Profiling of Yersinia pestis During Mouse Pulmonary Infection. DNA Cell Biol (2006).

  29. J Lawson, Stephen Johnston. Amplification of Sense-Stranded Prokaryotic RNA. DNA Cell Biol (2006).

  30. K Nalley, Stephen Johnston. Gal4 does not cycle on and off promoters while activating transcription. Nature (2006).

  31. S Chen, J Ding, R Bereredjian, B Yang, R Shohet, Stephen Johnston, H Hohmeier, C Newgard, P Grayburn. Efficient gene delivery to pancreatic islets with ultrasonic microbubble destruction technology. Proc. Natl. Acad. Sci (2006).

  32. C Solis, P Ostoa-Saloma, V Lugo-Martinez, Stephen Johnston, J Laclette. Genetic vaccination against murine cysticercosis by using a plasmid vector carrying Taenia solium paramyosin. Infection and Immunity (2005).

  33. C Solis, P Ostoa-Saloma, V Lugo-Martinez, Stephen Johnston, J Laclette. Related Articles, Links Genetic vaccination against murine cysticercosis by using a plasmid vector carrying Taenia solium paramyosin. Infect Immun (2005).

  34. D Magee, R Friedberg, M Woitaske, Stephen Johnston, R Cox. Role of B cells in vaccine-induced immunity against coccidioidomycosis. Infection and Immunity (2005).

  35. Jitakshi De, Ya-Ching Chang, Kausar Samli, Jonathan Schisler, Christopher Newgard, Stephen Johnston, Kathlynn Brown. Isolation of a Mycoplasma-specific binding peptide from unbiased phage-displayed peptide library. Molecular Biosystems (2005).

  36. K Stemke-Hale, B Kaltenboeck, F DeGraves, K Sykes, J Huang, C Bu, Stephen Johnston. Related Articles, Links Screening the whole genome of a pathogen in vivo for individual protective antigens. Vaccine (2005).

  37. K Stemke-Hale, B Kaltenboeck, F DeGraves, Kathryn Sykes, J Huang, C Bu, Stephen Johnston. Screening the whole genome of a pathogen in vivo for individual protective antigens. Vaccine (2005).

  38. Kausar Samli, Michael McGuire, Christopher Newgard, Stephen Johnston, Kathlynn Brown. Peptide Mediated targeting to the Islets of Langerhans. Diabetes (2005).

  39. A Talaat, R Lyons, S Howard, Stephen Johnston. The temporal expression profile of Mycobacterium tuberculosis infection in mice. PNAS (2004).

  40. Bao-Xi Qu, R Rosenberg, L Li, P Boyer, Stephen Johnston. Genetic vaccination to bias the immune response to amyloid beta peptide as therapy for alzheimers. Archives of Neurobiology (2004).

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Research Activity Research Awards

  1. Poste,George Henry * , Johnston,Stephen Albert , Lindsay,Stuart , Lindsay,Stuart , Stafford,Phillip , Woodbury,Neal Walter , Yan,Hao , Zhao,Zhan-Gong , . INSPIRE: Mimicking the Functional Complexity of Biology with Man-Made Systems. NSF-BIO (7/15/2012 - 6/30/2015).
  2. Johnston,Stephen Albert * , Papandreou-Suppappola,Antonia , Poste,George Henry , Stafford,Phillip , Woodbury,Neal Walter , . Detection of changes in health status and source of any infection using peptide array chips Technical PI: Stephen Johnston. DOD-DTRA (4/24/2012 - 12/31/2013).
  3. Johnston,Stephen Albert * . Serum Antibody Reactivity Profiling for Canine Lymphoma Detection and Monitoring. AKCCHF (1/1/2012 - 12/31/2012).
  4. Johnston,Stephen Albert * . Fc binding peptide development project. MILLIPORE WATERS (11/30/2011 - 10/16/2012).
  5. Fromme,Petra * , Allen,James Paul , Ghirlanda,Giovanna , Hogue,Brenda G , Johnston,Stephen Albert , Kumar,Sudhir , Labaer,Joshua , Leket-Mor,Tsafrir Shlomo , Ozkan,Sefika Banu , Sykes,Kathryn , Wachter,Rebekka M , Woodbury,Neal Walter , Poste,George Henry * , Johnston,Stephen Albert , Stafford,Phillip , Sykes,Kathryn , . Center for Membrane Proteins in Infectious Diseases MPID. HHS-NIH-NIGMS (7/1/2011 - 6/30/2015).
  6. Johnston,Stephen Albert * Diehnelt,Chris W Stafford,Phillip . Therapeutics to Any Pathogen in 7 Days. DOD-DARPA (7/15/2010 - 7/14/2012).
  7. Johnston,Stephen Albert * . Immunosignatures: a simple method to potentially diagnose Alzheimer's disease. ASU FDN (11/1/2009 - 10/31/2010).
  8. Johnston,Stephen Albert * . PREVENTING HIV BACKWARDS. GATES (BILL & MELINDA) FDN (10/1/2008 - 9/30/2009).
  9. Renaut,Rosemary Anne * Bimonte-Nelson,Heather Coon,David Wayne Johnston,Stephen Albert Marchant,Gary E Sierks,Michael Richard Ye,Jieping . AZ ASU Alzheimer's Reserach Center Project- Year 11. AADC (7/1/2008 - 6/30/2009).
  10. Johnston,Stephen Albert * Magee,Dewey Mitchell Stafford,Phillip . Peptide arrays for antigen identification and dianosis. UTMB (3/1/2008 - 2/28/2009).
  11. Johnston,Stephen Albert * . H.B. Wallace Research Initiative in the Center for Innovations in Medicine. WALLACE RSRCH FNDTN (1/1/2008 - 3/31/2009).
  12. Johnston,Stephen Albert * Lake,Douglas Fletcher . Towards Developing a Prophylactic Breast Cancer Vaccine. DOD-ARMY-USAMRAA (9/1/2007 - 6/30/2013).
  13. Johnston,Stephen Albert * Lake,Douglas Fletcher . Frameshifts as Cancer Vaccine Targets. KECK (WILLIAM) FDN (7/1/2007 - 6/30/2011).
  14. Renaut,Rosemary Anne * Aiken,Leona S Bimonte-Nelson,Heather Coon,David Wayne Farin,Gerald E Johnston,Stephen Albert Sierks,Michael Richard . AZ ASU Alzheimer's Research Center Project - Year 10. AADC (7/1/2007 - 6/30/2008).
  15. Alexander,Gene Evans * Bimonte-Nelson,Heather Coon,David Wayne Farin,Gerald E Johnston,Stephen Albert Renaut,Rosemary Anne Sierks,Michael Richard . AZ ASU Alzheimer's Research Center Project. AZ ADCC (7/1/2006 - 6/30/2007).
  16. Johnston,Stephen Albert * Magee,Dewey Mitchell Magee,Dewey Mitchell Sykes,Kathryn . Tularemia Vaccine Development Team. UNIV OF NEW MEXICO (3/2/2006 - 12/31/2011).
  17. Johnston,Stephen Albert * . Surface Plasmon Resonance Instrument for Biosignatures. DOD-ARMY-ARO (9/20/2005 - 9/19/2006).
  18. Johnston,Stephen Albert * Magee,Dewey Mitchell . Integration of Oncogenic Networks in Cancer Phenotypes. DUKE INSTITUTE/GENOME SCIENCES (6/1/2005 - 9/29/2009).
  19. Johnston,Stephen Albert * Magee,Dewey Mitchell . Region VI Center for Biodefense and Emerging Infections. UNIV OF TEXAS-GALVESTON (3/1/2005 - 2/29/2008).
  20. Johnston,Stephen Albert * Hoober,John Kenneth Joshi,Lokesh Joshi,Lokesh . ESTABLISHING A NEW AND NOVEL SYSTEM WITH POTENTIAL FOR HIGH IMPACT BIOMEDICAL USE: EXPLORING TRANSGENICALLY ENHANCED ROOT GROWTH SYSTEM. WALLACE RSRCH FNDTN (1/1/2001 - 6/30/2007).

* principal investigator

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Service

  1. Arnold and Mabel Beckman Foundation - Beckman Scholar Advisory Board, Advisory Board Member (2011 - present)
  2. Gates Grand Challenge Award, Review Panel Member (2010 - present)
  3. Gates Grand Challenge Award, Review Panel Member (2010 - present)
  4. Integration Panel DoD Breast Cancer Program, member (2008 - present)
  5. Integration Panel DoD Breast Cancer Program, member (2008 - present)
  6. Biological Design Graduate Program, Director, Co-founder (2007 - present)
  7. Biological Design Graduate Program, Director, Co-founder (2007 - present)
  8. EARS, member (2007 - present)
  9. EARS, member (2007 - present)
  10. Biodesign graduate effort, director (2005 - present)
  11. ad hoc on purchasing reform, leading (2005 - present)
  12. Forum on Microbial Threats, Intitute of Medicine of the National Academies, Member (2005 - present)
  13. National Center for Foreign Animal and Zoonotic Disease Defense, Member, External Advisor Board (2005 - present)
  14. Member, Institute of Medicine/ National Academy of Science Forum on Microbial Threats, Permenant member, advisor (2004 - present)
  15. Institute of Medicine/NAS Forum on Microbial Threats, Member (2004 - present)
  16. Institute of Medicine/NAS Forum on Microbial Threats, Member (2004 - present)
  17. SAB of Western Regional Center of Excellence for Biodefense and Emerging Infectious Diseases, SAB member (2003 - present)
  18. SAB of Western Regional Center of Excellence for Biodefense and Emerging Infectious Diseases, SAB member (2003 - present)
  19. DIA Biochem 20/20 ACE member, Founding Member of commt. to advise DoD on biothreats (1997 - present)
  20. BioChem 20/20 Advisory Committee of Experts, founding member (1997 - present)
  21. BioChem 20/20 Advisory Committee of Experts, founding member (1997 - present)
  22. President's Cancer Panel, Participant (2007)
  23. Department of Defense - Breast Cancer Research Program, Chair (2007)
  24. NIAID BPRC Programmatic Meeting, Keynote Speaker (2007)
  25. Maxygen HIVRAD, Advisory Board Member

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